Then, the XY stage is commanded to move the center of the home image so that it can be imaged at each of four corners of the display. A similar image series is acquired and analyzed along the Y axis. A cross correlation between the last displaced image and the initial home image is used to measure the size of the displacement between them in pixels. An image is acquired with the current hardware settings at each new position until the stage has moved by nearly a whole field of view. Then it doubles the displacement for each subsequent move. The Pixel Calibrator plugin acquires a first “home” image, and then commands the XY stage to move along the X axis by a very small initial displacement (1 micron). Press the “Start” button to initiate pixel calibration. We assume that the reader has a basic understanding of the use of μManager, as described previously. Here, we present step-by-step instructions on how to use a number of advanced features and plugins that have been added to μManager in recent years. Furthermore, μManager’s plugin capabilities allow new application-level functionality to be added for specialized techniques. Likewise, vendor-neutral command sets are provided for shutters, stages, and multi-state devices such as objective turrets and filter wheels. The application’s programming interface for hardware control provides a common set of commands that can be used to control a supported camera from any manufacturer. ΜManager’s design facilitates both routine applications and the development of advanced microscopy methods. The routine use of μManager is extensively documented, both on the μManager website and in a previous publication. μManager is currently used in thousands of laboratories worldwide and is mentioned in more than 500 publications. μManager runs as an ImageJ plugin (ImageJ is a widely used, freely available image analysis software ), and can be downloaded at no cost from the website. The application provides an intuitive graphical user interface and a documented programming interface for controlling microscope hardware. ΜManager (“Micro-Manager”) is an open-source, cross-platform (Windows, Mac, and Linux) desktop application that facilitates computer control of light microscopes for researchers.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |